Deficient Biosynthesis of N - Acetylglucosaminyl - Phosphatidylinositol , the First hte ~ iiiediate of Glycosyl Phosphatidylinositol Anchor Biosynthesis , in Cell Lines
نویسندگان
چکیده
Deficient Biosynthesis of N-AcetylglucosaminylPhosphatidylinositol, the First hte~iiiediate of Glycosyl Phosphatidylinositol Anchor Biosynthesis, in Cell Lines Established from Patients with Paroxysmal Nocturnal Hemoglobinuria By Minoru Takahashi,* Junji Takeda,* Shinichi Hirose,$ Robert Hyman, ll Norimitsu Inoue,* Toshio Miyata,* Etsuko Ueda,~S Teruo Kitani,~ M. Edward Medof,$ and Taroh Kinoshita*
منابع مشابه
Deficient Biosynthesis of N - Acetylglucosaminyl - Phosphatidylinositol , the First hte ~ iiiediate of Glycosyl Phosphatidylinositol Anchor Biosynthesis , in Cell Lines Established from Patients with Paroxysmal Nocturnal Hemoglobinuria
Deficient Biosynthesis of N-AcetylglucosaminylPhosphatidylinositol, the First hte~iiiediate of Glycosyl Phosphatidylinositol Anchor Biosynthesis, in Cell Lines Established from Patients with Paroxysmal Nocturnal Hemoglobinuria By Minoru Takahashi,* Junji Takeda,* Shinichi Hirose,$ Robert Hyman, ll Norimitsu Inoue,* Toshio Miyata,* Etsuko Ueda,~S Teruo Kitani,~ M. Edward Medof,$ and Taroh Kinosh...
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Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic disorder caused by a deficiency of biosynthesis of the glycosyl phosphatidylinositol (GPI) anchor, but the biochemical defect is not completely understood. In the present study, we have analyzed affected cell lines established recently from two Japanese patients with PNH. Two lines of evidence indicate that these cells do not synthesize N...
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Many eukaryotic cell surface proteins are bound to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. Several genes involved in GPI anchor biosynthesis have been cloned using complementation of mutant mammalian cell lines and yeasts that are defective in its biosynthesis pathway. However, the gene involved in the second step of this pathway, in which N-acetylglucosaminyl-phosphat...
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Non-acetylated glucosamine is an unusual structural feature shared by all glycosyl phosphatidylinositol (GPI) lipids, including a variety of membrane anchors, the leishmanial lipophosphoglycan, and a mediator of insulin action. We proposed previously a pathway for biosynthesis of glycolipid A, the precursor of the GPI membrane anchor of the trypanosome variant surface glycoprotein (Masterson, W...
متن کاملEarly lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesized in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer
Glycosylated phosphoinositides serve as membrane anchors for numerous eukaryotic cell surface glycoproteins. Recent biochemical and genetic studies indicate that the glycolipids are assembled by sequential addition of components (monosaccharides and phosphoethanolamine) to phosphatidylinositol. The biosynthetic steps are presumed to occur in the ER, but formal proof of this is lacking. We descr...
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